Simple permanent tissues

=Activity No # 1 -Observation of simple permanent tissues=

Estimated Time
30 minutes

Materials/ Resources needed
A piece of stem, blade, dissection needle, a microscope, glass slide, cover slip, glass dish, methylene blue, glycerine, blotting paper etc.

Prerequisites/Instructions, if any
Cut very thin sections of a soft stem and select a good section and observe it under microscope.Note the different types of cells you observe.

Process (How to do the activity)

 * Cut a soft piece of a plant stem(shrub) of 1 to 2 inches in length.
 * Take very thin sections(10 to 20) of the stem and place it in a glass dish with water.
 * Select a very fine section and place it on a glass slide.
 * Add 1 to 2 drops of methylene blue on the section and wash the extra stain with water.
 * Add 1 to 2 drops of glycerine and cover the section with a cover slip gently and remove the extra stain with a blotting paper.
 * Mount the slide on the microscope.

Developmental Questions (What discussion questions)

 * 1) How many types of cells do you observe?
 * 2) Do the different types of cells differ in their structure?
 * 3) Why do they differ in structure?
 * 4) How many types of simple permanent tissues can you observe?

Evaluation (Questions for assessment of the child)

 * 1) How can you differentiate parenchyma from collenchyma cells?
 * 2) How can you differentiate sclerenchyma from collenchyma cells?

Question Corner
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