*Take very thin sections(10 to 20) of the leaf and place it in a glass dish with water.
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*Break the leaf by stretching it so that a peel of skin from the lower epidermis projects out.Remove 2 to 3 pieces of peel and place it in a glass dish with water.
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*Select a very fine section and place it on a glass slide.
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*Select a very fine peel and place it on a glass slide.
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*Add 1 to 2 drops of methylene blue on the section and a drop of glycerine.
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*Add 1 to 2 drops of methylene blue on the peel and a drop of glycerine.
*Cover the section with a cover slip gently and remove the extra stain with a blotting paper.
*Cover the section with a cover slip gently and remove the extra stain with a blotting paper.