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From Karnataka Open Educational Resources
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*Take very thin sections(10 to 20) of the stem and place it in a glass dish with water.
 
*Take very thin sections(10 to 20) of the stem and place it in a glass dish with water.
 
*Select a very fine section and place it on a glass slide.
 
*Select a very fine section and place it on a glass slide.
*Add 1 to 2 drops of methylene blue on the section and a drop of glycerine.
+
*Add 1 to 2 drops of methylene blue on the section and wash the extra stain with water.
*Cover the section with a cover slip gently and remove the extra stain with a blotting paper.
+
*Add 1 to 2 drops of glycerine and cover the section with a cover slip gently and remove the extra stain with a blotting paper.
 
*Mount the slide on the microscope.
 
*Mount the slide on the microscope.